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Unlocking CRISPR with AI Tutoring: A New Learning Approach

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Chapter 1: Introduction to CRISPR Tutoring

In this course, we introduce learners to CRISPR through the lens of AI tutoring, leveraging structured data to enhance the educational experience. Utilizing the advanced capabilities of ChatGPT-4 Turbo, our aim is to establish a "Learn to Earn Academy" on Ascendance.dev. Here, users can earn digital and web 3 tokens by engaging with the course material.

This video provides an overview of the CRISPR Tutor Custom GPT and its functionalities.

Futuristic CRISPR laboratory concept design

Chapter 2: Interactive Learning Levels

To facilitate effective learning, we offer four conversation starters within the GPT framework. These levels cater to varying degrees of familiarity with CRISPR:

  1. CRISPR Level: Beginner
    • Key Topics: CRISPR-Cas9, gRNA Design, Cas9, Genome Editing, Off-Target Effects, Homology Directed Repair (HDR), Non-Homologous End Joining (NHEJ)
  2. CRISPR Level: Intermediate
    • Key Topics: PAM Sequence, Cas12 and Cas13, Base Editing, Prime Editing, Gene Drive, CRISPR Screening, CRISPR Therapeutics
  3. CRISPR Level: Advanced
    • Key Topics: Multiplex Genome Editing, CRISPR Activation (CRISPRa) and Interference (CRISPRi), High-Fidelity Cas Variants, Epigenome Editing with CRISPR, CRISPR Diagnostic Tools, CRISPR Ethical Considerations, CRISPR in Agriculture

Learn how the Learn to Earn Academy utilizes Custom GPT for effective tutoring.

Chapter 3: Understanding CRISPR Concepts

To provide clarity on essential CRISPR concepts, we outline the following definitions:

  • CRISPR: A collection of DNA sequences found in prokaryotes, originating from segments of bacteriophage DNA.
  • Cas9: An enzyme that utilizes CRISPR sequences to identify and cut specific DNA strands.
  • CRISPR-Cas9: A gene-editing technology that employs the Cas9 enzyme alongside CRISPR sequences.
  • Antiviral Defense System: A protective mechanism in prokaryotes involving CRISPR sequences against bacteriophages.
  • Acquired Immunity: A form of immunity in prokaryotes attributed to CRISPR sequences, safeguarding against bacteriophages.

For a comprehensive understanding of CRISPR's functionalities, including its various applications in gene editing and diagnostics, consult our CRISPR GPT Tutor powered by ChatGPT-4 technology.

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